MS 6 22 2017
Culture 1518 T3 ligase 200ml.
Culture 1697 pCold-GST-(3c)-wt3C 8L, induced with IPTG at 16oC.
Spin down and homogenize 1613 pET28-cold-GST-3C 8L, load onto Ni column.
Wash Ni column (load with 1942 phi29) with Ni washing Buffer, Tris-HCl, Buffer B.
Elute QHP column (load with 1518 T3 ligase)
Run SDS-PAGE gel of 1518 T3 ligase 5.62 / 41.6 06/22/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
|
sample |
11 |
12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
MW |
|
uL |
15 |
15 |
15 |
15 |
15 |
15 |
15 |
15 |
15 |
10 |
forward lane 7~8 to the next step.
Run SDS-PAGE gel of 2295 yPolyA 7.63 / 66.2 06/22/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
|
sample |
20 |
21 |
22 |
23 |
24 |
25 |
26 |
MW |
|
uL |
4.6 |
0.8 |
0.5 |
1.0 |
1.2 |
1.3 |
1.5 |
10 |
|
ddH2O |
10 |
15 |
15 |
15 |
15 |
15 |
15 |
\ |
forward lane 1~3 to the next step.