MS 6 29 2017
Culture 2462 Tn5 transposase 200ml.
Culture 1811 vPol 8L, induced with IPTG.
Homogenize 1995 Kfu, load onto Ni column.
Wash Ni column (load with 1458 KOD) with Ni washing Buffer/ Tris-HCl/ Buffer B, store at -80oC.
Elute 1458 KOD with Buffer B 06/29/2017
| -0.02 |
-0.00 |
-0.01 |
0.09 |
0.12 |
(1~5) |
| 4.98 |
18.72 |
18.77 |
11.26 |
6.98 |
(6~10) |
| 4.54 |
2.84 |
1.89 |
1.31 |
0.92 |
(11~15) |
| 0.72 |
0.58 |
0.51 |
0.44 |
0.39 |
(16~20) |
| 0.24 |
0.16 |
0.13 |
0.07 |
0.04 |
(21~25) |
collect 6~21.
Elute 1518 T3 ligase from QHP column.
Run SDS-PAGE gel of 1518 T3 ligase 5.62/41.6 06/29/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
sample |
26 |
28 |
29 |
30 |
31 |
32 |
33 |
34 |
MW |
35 |
36 |
37 |
|
uL |
6.7 |
4.8 |
4.2 |
3.4 |
3.5 |
2.5 |
2.0 |
1.8 |
10 |
1.9 |
2.6 |
3.7 |
|
ddH2O |
10 |
10 |
10 |
10 |
10 |
10 |
15 |
15 |
\ |
15 |
10 |
10 |
forward lane 10~12 to the next step.