MS 6 30 2017
Spin down 1811 vPol and store at -20oC.
Wash Ni column (load with 1995 Kfu) with Ni washing Buffer/ Tris-HCl/ Buffer B, store at -80oC.
Elute 1995 Kfu with Buffer B 06/30/2017
| -0.21 |
-0.19 |
-0.09 |
0.02 |
1.26 |
(1~5) |
| 1.09 |
0.85 |
0.69 |
0.58 |
0.58 |
(6~10) |
| 0.58 |
0.56 |
0.55 |
0.54 |
0.51 |
(11~15) |
| 0.45 |
0.40 |
0.40 |
0.36 |
0.33 |
(16~20) |
| 0.32 |
0.30 |
0.21 |
0.14 |
0.08 |
(21~25) |
collect 5~22.
Prepare LB media 20L.
Dialysis of 1942 phi29, 1697 GST-wt3C, 1518 T3 ligase.
1942 phi29: 1.07mg/ml, 3.0mg, purity >90%.
1697 GST-wt3C: 3.03mg/ml, 4.0mg, purity >90%.
1518 T3 ligase: 2.50mg/ml, 3.5mg, purity >90%.