MS 7 4 2017
Culture 423 pET30b-DeepVent-sso7d 200ml.
Culture 1567 Exo 8L, induced with IPTG.
Homogenize 2462 Tn5 transposase, load onto Ni column.
Load 1995 Kfu onto QHP column.
Wash Ni column (load with 1811 vPol) with Ni washing buffer/ Tris-HCl/ Buffer B.
Elute 1811 vPol with Buffer B 07/04/2017
| -0.02 |
-0.11 |
-0.14 |
-0.21 |
-0.08 |
(1~5) |
| 1.18 |
3.41 |
6.35 |
17.97 |
36.35 |
(6~10) |
| 20.81 |
15.44 |
10.71 |
4.83 |
1.61 |
(11~15) |
| 0.76 |
0.32 |
0.24 |
0.12 |
0.08 |
(16~20) |
collect 6~17.
Elute 1458 KOD
Run SDS-PAGE gel of 1458 KOD 7.91/92.2 07/04/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
sample |
16 |
18 |
20 |
22 |
24 |
26 |
28 |
30 |
MW |
32 |
34 |
36 |
|
uL |
10 |
0.2 |
0.1 |
0.13 |
0.13 |
0.2 |
0.23 |
0.7 |
10 |
2.0 |
4.2 |
7.7 |
|
ddH2O |
5 |
15 |
15 |
15 |
15 |
15 |
15 |
15 |
\ |
15 |
10 |
5 |
forward lane 1~3 to the next step.