MS 7 5 2017
Culture 1782 Tth Ligase 200ml.
Culture 423 pET30b-DeepVent-sso7d 8L, induced with IPTG.
Homogenize 1567 Exo, load onto Ni column.
Load 1811 vPol onto QHP column.
Wash Ni column (load with 2462 Tn5 transposase) with Ni washing buffer/ Tris-HCl/ Buffer B.
Elute 2462 Tn5 transposase with Buffer B 07/05/2017
| -0.24 |
-0.26 |
-0.24 |
-0.23 |
-0.20 |
(6~10) |
| -0.15 |
0.28 |
1.31 |
0.38 |
0.04 |
(11~15) |
| 0.00 |
(16~20) |
collect 12~15.
Elute 1995 Kfu
Run SDS-PAGE gel of 1995 Kfu 7.87 / 99.3 07/05/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
sample |
17 |
18 |
19 |
20 |
21 |
22 |
23 |
MW |
25 |
27 |
29 |
31 |
|
uL |
9.1 |
1.5 |
0.8 |
0.7 |
1.1 |
1.7 |
2.4 |
10 |
3.3 |
4.4 |
6.7 |
11.1 |
|
ddH2O |
5 |
15 |
15 |
15 |
15 |
15 |
15 |
\ |
10 |
10 |
10 |
5 |
forward lane 1~4 to the next step.
Dialysis of 1458 KOD. 14.27mg/ml, 4.5ml, purity > 90%