MS 7 11 2017
Homogenize 818 pHis-GST-His-Flag-His, centrifugate and load onto Ni column.
Wash Ni column (load with 1782 Tth-Ligase) with Ni washing buffer/ Tris-HCl/ Buffer B. Elute 1782 Tth-Ligase with Buffer B 07/11/2017
| -0.26 |
-0.27 |
-0.28 |
-0.19 |
0.03 |
(1~5) |
| 4.68 |
6.36 |
7.35 |
5.35 |
4.74 |
(6~10) |
| 4.15 |
3.85 |
3.53 |
2.75 |
2.35 |
(11~15) |
| 1.85 |
1.53 |
1.21 |
0.54 |
0.23 |
(16~20) |
| 0.12 |
0.08 |
(21~25) |
collect 6~20.
Load 423 DeepVent-sso7d onto QHP column.
Elute 1567 T5-Exo
Run SDS-PAGE gel of 1567 T5-Exo 5.55 / 34.5 07/11/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
sample |
19 |
20 |
21 |
22 |
23 |
24 |
25 |
MW |
26 |
27 |
28 |
30 |
|
uL |
9.1 |
5.3 |
3.9 |
3.0 |
3.1 |
2.6 |
2.4 |
10 |
3.3 |
4.8 |
5.3 |
9.1 |
|
ddH2O |
5 |
10 |
10 |
10 |
10 |
10 |
10 |
\ |
10 |
10 |
10 |
5 |
Forward lane 11~12 to the next step.