3023 GST-VIII

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in pJet1.2 vector, miniprep digest with Xba1 + Xho1

subcloning

subclone into 433, digest plasmid wit Xba1 + Xho1, wrong

should digest with EcoR1 + Xho1

digest with EcoR1+ Xho1

Need to do PCR to confirm

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Cloning