2196-pT7A3E-PBCV-1-Lig
Cloning of 2196-pT7A3E-PBCV-1-Lig
[edit]Purpose: Cloning of 2196-pT7A3E-PBCV-1-Lig
Date: 2014-5-5---2014-5-8
Notebook:0
Page:
Clone ID:2196
Experimenters: yingying
Results
[edit]cloning
[edit]
gene synthesis
PBCV-1-Lig-1 to PBCV-1-Lig-24
| lane |
3 |
4 |
| PCR |
1892 pT7A3E | |
| Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2014-5-8
Make miniprep and digest with Nde1 + Xho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]Fix of 2196-pT7A3E-PBCV-1-Lig
[edit]Purpose: fix of 2196-pT7A3E-PBCV-1-Lig
Date: 2014-5-12---2014-5-14
Notebook:0
Page:
Clone ID:2196
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1: tmplate 2196-1
PBCV-1-Lig-1+PBCV-1-Lig-10
pcr2: tmplate 2196-2
PBCV-1-Lig-11+PBCV-1-Lig-22
pcr3: tmplate 2196-1
PBCV-1-Lig-23+PBCV-1-Lig-23
PCR:template:pcr1+pcr2+pcr3
PBCV-1-Lig-1+PBCV-1-Lig-24
| lane |
3 |
4 |
| PCR |
1892 pT7A3E | |
| Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2014-5-15
Make miniprep and digest with Nde1 + Xho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]Product info
[edit]http://molbiol.edu.ru/eng/scripts/01_04.html
1 reaction require 8 pmol enzyme, 34k Da, 1 µl (25U), 1 pmole=34 ng, 272 ng--> 8 pmol.
1 µl -->25 U--8 pmole--> 272 ng1,000 Units/25=40 ul. X272 ng =10 ug /0.9 (90% purity)=12 ug.