2031 2032

Protein Expression

2014/1/14

PageRuler Plus 10, 15, 25(red), 35, 55, 70(red), 100, 130, 250
Paul

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw
1	pCold-Tat-yFLP	Tat-yFLP	2031	5 ml, Nico21(DE3)pRare2pLysS	1 mM IPTG  RT o/n	pellet, dissolved in 8M Urea (Insoluble fraction) Ni beads	9.76 / 52.8
2	pCold-Tat-yFLP	Tat-yFLP	2031	5 ml, Nico21(DE3)pRare2pLysS	1 mM IPTG RT o/n	Soluble fraction of cell lysate Ni beads	9.76 / 52.8
3	pCold-Tat-yFLP	Tat-yFLP	2031	5 ml, Nico21(DE3)pRare2pLysS	1 mM IPTG RT o/n	pellet, dissolved in 8M Urea (Insoluble fraction) Ni beads	9.76 / 52.8
4	pCold-Tat-yFLP	Tat-yFLP	2031	5 ml, Nico21(DE3)pRare2pLysS	1 mM IPTG RT o/n	Soluble fraction of cell lysate Ni Magnetic beads	9.76 / 52.8
5	PageRuler Plus Prestained protein ladder
6	pCold-Tat-Flpo	Tat-Flpo	2032	5 ml, Nico21(DE3)pRare2pLysS	1 mM IPTG RT o/n	pellet, dissolved in 8M Urea (Insoluble fraction) Ni beads	9.76 / 52.8
7	pCold-Tat-Flpo	Tat-Flpo	2032	5 ml, Nico21(DE3)pRare2pLysS	1 mM IPTG RT o/n	Soluble fraction of cell lysate Ni beads	9.76 / 52.8
8	pCold-Tat-Flpo	Tat-Flpo	2032	5 ml, Nico21(DE3)pRare2pLysS	1 mM IPTG RT o/n	pellet, dissolved in 8M Urea (Insoluble fraction) Ni beads	9.76 / 52.8
9	pCold-Tat-Flpo	Tat-Flpo	2032	5 ml, Nico21(DE3)pRare2pLysS	1 mM IPTG RT o/n	Soluble fraction of cell lysate Ni Magnetic beads	9.76 / 52.8

Ni megatic beads are not good. 2031 pCold-Tat-yFLP has better expression than 2032 pCold-Tat-Flpo