2085 2101 2146 2163 2187+2188 2190 2195 2203 20140512
Protein Expression
[edit]2014/5/12
SHANYI
MBP tag MW: 42 Kd
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pT7A3-MBP-TRX-sso7d-6H |
MBP-TRX-sso7d-6H |
2085 |
15 ml, Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.44/63.7 |
YES |
| 2 |
pT7A3-sso7d |
sso7d |
2101 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
9.33/9.8 |
yes |
| 3 |
pT7A3HC-6H-Tat-sc-MS2 |
-MS2 |
2146 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
30.5 |
yes |
| 4 |
pageRuler Plus Prestained protein ladder |
|||||||
| 5 |
pT7A3E-HC-Tma-Endo-IV |
HC-Tma-Endo-IV |
2163 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.65/32.43 |
yes |
| 6 |
pT7A3E-HC-Tma-Endo-IV |
-HC-Tma-Endo-IV |
2163 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
p |
yes | |
| 7 |
pT7A3HC-6H-HA2-R9 |
6H-HA2-R9 |
2187 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
10.65/5.58 |
|
| 8 |
pT7A3E-6H-HC-Tat-HA |
6H-HC-Tat-HA |
2188 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
10.84/4.49 |
|
| 9 |
pT7A3E-6H-Tth-SOD |
6H-Tth-SOD |
2190 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.62/24.5 |
yes |
| 10 |
pT7A3E-Vac-DNA-Ligase |
Vac-DNA-Ligase |
2195 |
15 ml,
Tuner(DE3)plysN |
0.3mM IPTG RT O/N |
S |
7.78/63.36 |
YES |
| 11 |
pCold-MBP-Tat-NLS-iFLP-6H |
MBP-Tat-NLS-iFLP-6H |
2203 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
9.03/96.9 |
|
| 12 |
pCold-MBP-Tat-NLS-iFLP-6H |
2203 |
15 ml,
Nico21(DE3)plysN |
p |
yes insoluble |