2106 2115 cre
PageRuler Plus 10, 15, 25(red), 35, 55, 70(red), 100, 130, 250
| lane |
1 |
2 |
3 |
4 |
5 | |||||
| clone ID |
2106 pET28b-iFLP |
2115 pT7A3-iFLP |
PageRuler Plus Prestained protein ladder |
1708+1823 | ||||||
| host |
Nico21DE3plysN |
Nico21DE3plysN |
Nico21DE3plysN | |||||||
| induction |
0.2 mM IPTG 25 oC o/n 5ml |
0.2 mM IPTG 25 oC o/n 5ml |
||||||||
| purification |
Ni beads |
Ni beads |
Ni Column-- SpFF--SpFF | |||||||
| MW |
pI/Mw: 9.76 / 52.8 |
pI/Mw: 9.76 / 52.8 |
10.30 / 42.8 | |||||||