1949 pT7A3-Kfu-sso7d
Cloning of 1949 pT3A3-Kfu-sso7d
[edit]Purpose: Cloning of 1949 pT3A3-Kfu-sso7d
Date: 2013-12-4---
Notebook:0
Page:
Clone ID:1949
Experimenters: shanyi
Results
[edit]cloning
[edit]PCR
PCR two fragments from Kod 1801 pT7A30-KOD-sso7d
1
Kfu-F1+Kfu-R2
2
Kfu-F3 c+Kfu-R4
PCR 1 fragment from Pfu 1715 pT7A30-Pfu-sso7d
3
Kfu-F2+Kfu-R3
Join three fragments using
Kfu-F1+Kfu-R4
digest with Nde1 + Xho1 and clone into Nde1 + Xho1 digested vector 1847 pT7A30-sso7d-6H
Colony Screening
[edit]Date: 2013-12-6
Make miniprep, digest with NdeI+XhoI
send 1949-2 to sequence with T7term primer