Packagingmix optimization 1
1 Day after transfection
2 days after transfection
| Vector |
Packaging mix |
Transfection Reagent | |
| 1 |
- |
- |
- |
| 2 |
- |
Sigma |
PolyExpress |
| 3 |
pLL3.7 |
Sigma |
PolyExpress |
| 4 |
pLL3.7 |
1:1 |
PolyExpress |
| 5 |
pLL3.7 |
1:3 |
PolyExpress |
| 6 |
pLL3.7 |
1:5 |
PolyExpress |
| 7 |
pLL3.7 |
1:10 |
PolyExpress |
| 8 |
pSMPUW-EGFP |
Sigma |
PolyExpress |
| 9 |
pSMPUW-EGFP |
1:3 |
PolyExpress |
| 10 |
pSMPUW-EGFP |
Sigma |
PolyExpress |
| 11 |
pSMPUW-EGFP |
1:3 |
FuGene |
| 12 |
pSMPUW-EGFP |
Sigma |
FuGene |
Fugene: 1.2 ul
PolyExpress: 4.5 ul
pLL3.7:0.2 ug (1ul of 200 ng/ul)
pSMPUW-EGFP: 0.2 ug (1ul of 200 ng/ul)
Packaging mix: 2 ul (Sigma or homemade, pVSVG/pGag-Pol)
add 1 ml conditioned medium (containinng lentivirus) to cells
1 day post infection --Paul.xu 12:54, 29 January 2011 (EST)
2 days post infection
--Paul.xu 10:46, 30 January 2011 (EST)
3 days post infection
--Paul.xu 11:13, 1 February 2011 (EST)
5 days post infection
--Paul.xu 10:49, 3 February 2011 (EST)
6 days post infection
--Paul.xu 09:39, 4 February 2011 (EST)