Ligation of DNA fragment and pMiniT2
1.Contratc out with company to synthsize DNA fragments.
2.See the instruction of the related DNA fragments.
3.Use 10ul to disolve the Samples.
4.Transfer 1 ul to ligate with pMiniT2 Vector.
5.Formula:
a.1 ul diluted DNA fragments+3 ul H2O
b.0.5 ul pMiniT2 vector
c.4ul Cloning Mix 1(buffer)
d.1 ul Cloning Mix 2(Ligase)
6.Ligate at RT for 15 min.
7.Then do transformation.