Yijia LI 2 28 2018

Date:2/28/2018

Miniprep digestion for 3154_3129

Digestion buffer:Fastdigest

Restriction enzymes:

3154:BamH I+ Kpn Iinsert length:527

3129:Kpn I+Not Iinset length:1034

Result:seems good, chose 3129-1 to do sequencing

Date:2/27/2018

pCEL0

Date:2/28/1028

Digestion of 3129 vector with two enzymes:

BsrG I+Kpn I, BsrG I only, Kpn only

Buffer NEB 2

 Volume of vector:0.5 ul

Date:3/1/2018

Test for 3129-pCMV5-HA-VH-mIgG2b digestion sites:
1:Sac I +BsrG I
2:Sall+BsrG I
3:Xho I+ BsrG I
4:BsrG I
5:Kpn I+BsrG I
Volume of DNA:1 ul
Buffer:Fastdigest 
Time:30mins