3010 pCMV5-Flag-kappa

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http://www.excellgen.net/eln/images/2/2f/3001_3010_pMiniT2_digestion.JPG


2/4/2018

3010 pCMV5-Flag-kappa, Flag light
need to fix from 3135-4-pMiniT2 >as-F_E12.ab1 template
it is actually 3010: missing 
5' end ATCATCGGATCCGCC
3' end: GGAGGAACAAAGCTCGAGATCAAGCGGGCTAGCGGTACCATCATC
using these oligos
1 ul of 10 uM
3010-Flag-VL-1C4ATCATCGGATCCGCCGCCACCATG
1 ul of 1 uM
3010-Flag-VL-2C5AGAGCAGCAGGAGCCCCAGGAGCTGAGCGGGGACCCTCATGTCCATGGTGGCGGCGGA
1 ul of 1 uM
3010-Flag-VL-11D2CGCTCCTTACACCTTTGGCGGAGGAACAAAGCTCGAGATCAAGCGGGCTAGCGGTACC
1 ul of 10 uM
3010-Flag-VL-12D3GATGATGGTACCGCTAGCCCGC
3135-4-pMiniT2 as template


Vector Name: 2493 pCMV5-mIgG-kLC
Restriction Enzymes: BamH1-Kpn1

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3010 miniprep:

Restriction enzymes: BamHI+KpnI

Time:15min

Buffer:Fastdigest

Result:good, sent to do sequencing.#4

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