1834-pAvi
Cloning of 1834-pAvi
[edit]Purpose: Cloning of 1834-pAvi
Date: 2013-10-8----2013-10-11
Notebook:0
Page:
Clone ID:1834
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR from BL21 genomic DNA using these 3 oligos:
1ul of 10 uM of BirA-HindIII-F1
1ul of 1 uM of BirA-F2
1ul of 10 uM of BirA-Sal1-R
| lane |
1 |
2 |
| PCR product |
1727,1727 pT7A30 | |
| HindIII + Sal1 |
HindIII + Xho1 |
Colony Screening
[edit]Date: 2013-10-11
Make miniprep and digest with xba1+xho1
digest with nde1+pst1
Miniprep of 945pMiniT2_947pMiniT2_3131_1727_1834
945pMiniT2:1-4 EcoRI
947pMiniT2:5-8 n EcoRI
3131:9-13 BssHII+XhoI
1727:14 XbaI+NcoI
1834:15 XbaI+NcoI
Buffer:Fastdigest
Time:30Min
Results:945pMiniT2 and 947pMiniT2 are good, so sent them to do sequencing. 3131 is not good, 1727 has no band, 1834 has one band.
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Result intepretation/Summary
[edit]Conclusion
[edit]