3167 pCEH0-Myc-VH-mIgG2a

Date:3/12/2018

subclone of 3167:

Restriction enzymes:BsrGI+NheI

Buffer:Fastdigest

Time:15 min at 37oC

Result: Vector is good, insert is not good, just cut out vector

Date:3/14/2018 Miniprep digestion of 3131_3161_3167

Name length Enzymen Buffer

3131:1-4 342 BssHII+XhoI Cutsmart

3161:5-8 225 NdeI+XhoI Cutsmart

3167:9-12 397 BsrGI+NheI NEB 2

Time:40min

Redo miniprep of 3131_3161_3167

3131:1-4 BssHII+XhoI 342 Cutsmart

3161:5-8 NdeI+XhoI 225 Cutsmart

3167:9-12 BsrGI+NheI 397 NEB 2

Time:40min

Redo Subclone of 3167

Vector:3163pCEH0

Insert:3027pMiniT2

BsrGI+KpnI

Buffer:NEB 2

Time:30min

Result:Cut out and combine

Date:3/16/2018

Miniprep of 945_3167

945:1-4 Xba+NcoI Cutsmart 1326

3167:5-8 BsrGI+NheI NEB 2 397

Time:50min

Result: