Transfection with MiniPrep DNA

1, Split 293T cells into 12-well plate 24 hours before transfection.

2, On next day, cells should be 70%~90% conflucence.

3, Add 50 µl of DMEM without serum to 1.5 ml tube.

4. Take 5 µl of plasmid DNA (~0.5 to 1 µg DNA), add to the tube (step 3).

5. Make transfection reagent mix.

   For each sample,  add 1 to 3 ul of transfection reagent to 50 µl of DMEM without serum.

   DNA to transfection reagent ratio is 1:3.

6. Add 50 µl of transfection reagent mix to each tube, mix gently.

7. Incubate at room temperature for 15 to 20 min.

8. Add 100 µl mixture to each well.