Yijia LI 3 26 2018
Date:3/26/2018
[edit]Subclone of 947
Vector:1727
Insert:947pMiniT2---#3
XbaI+NcoI-HF
Subclone of 945
Vector:1727
Insert:945pMiniT2
XbaI+NcoI-HF
Test of 947_945_3166_3165
| Name | Enzymes | Size (bp) | Lane |
| 947 | BglII | 646 | 1,2,3 |
| BssHII | 1856 | 4,5,6 | |
| 945 | BglII | 628 | 11,12 |
| BssHII | 1838 | 13 | |
| 3165 | SalI+BsrGI | 693 | 8 |
| HindIII+BsrGI | 441 | 10 | |
| 3166 | SalI+BsrGI | 1234 | 9 |
| XbaI | 2049 | 7 | |
| BglII | 3259 | to do |
PCR of 3127
3127 PCR
3097 as template
3127-NdeI-F
3127-HindIII-R
408 bp
extension:30s
Subclone of 3165
Vector:3129
Insert:3165pMiniT2
SalI+BsrGI
Date:3/27/2018
[edit]Test of miniprep digestion
3166: lane 1-3, SalI+BsrGI 1234bp
3166: lane 4-6, XbaI 2049bp
3166: lane 7-9, BglII 3259bp
3165: lane 10,11, SalI+BsrGI 693bp
3165: lane 12,13, HindIII+BsrGI 441bp
redo 3152 subclone
Subclone of 3122
subclone of 939
subclone of 938
Size:1145bp
V:1987 pT7A3
Date:3/28/2018
[edit]
Date:3/29/2018
[edit]Miniprep of 3152 digestion
1-4:samples
+:control for 3009pminiT2
Enzymes:BsrGI+NheI
Miniprep of 3127p_3166_973-2_973-1 digestion
3127pMiniT2:1-4
3166pMiniT2:5-8
973-2pMiniT2:9-12
973-1pMiniT2:13-16
Enzymes:EcoRI
3166 itself has EcoRI sites
Date:3/30/2018
[edit]Miniprep digestion of 3122_938_939
3122:lane 1-4 lane 5 :+ size:1743 bp
938: lane 6-9 lane 10:+ size:1145 bp
939: lane 11-14 lane 15:+ size:780 bp
Positive control are insert-pjet1.2
The small band size of vector 1727:193 bp
redo Miniprep digestion of 3122_938_939
1:3122 size:1743bp
2:3122pjet1.2 sequenced +
3:938 size:1145 bp
4:938pjet1.2 sequenced +
5,6,7:939 size:780 bp
8:939pjet1.2 sequenced +
Enzymes:NdeI+XhoI
Date:4/1/2018
[edit]Miniprep digestion of 973-2pMiniT2
Enzymes:EcoRI
Size:500bp
