Hybridoma Bioreactor Culture

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Top chamber:
DMEM
https://www.thermofisher.com/order/catalog/product/11995065?SID=srch-hj-11995-065
 
(5%) CDM HD serum replacement
http://www.fibercellsystems.com/products/cdm-hd-chemically-defined-high-density-serum-replacement/
 Top chamber medium: DMEM, high glucose, pyruvate  Catalog number:  11995065 
Add (5%) CDM HD as serum replacement
 
Bottom chamber
Hybridoma-SFM
https://www.thermofisher.com/order/catalog/product/12045076?SID=srch-hj-12045-076
 
5% Corning Fetal Bovine Serum, Premium (Low IgG)
https://www.fishersci.com/shop/products/corning-cellgro-low-igg-fbs-media-1-x-500ml/mt35073cv
 
Hybridoma-SFM Catalog number:  12045076 
Corning Fetal Bovine Serum, Premium (Low IgG)
 
Corning Celline Bioreactor
https://www.fishersci.com/shop/products/corning-celline-disposable-bioreactor-celline-disposable-reactor/101264
 
Wheaton Celline Bioreactors
https://www.fishersci.com/shop/products/wheaton-celline-bioreactor-flask-8/p-4485893

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