997 pT7A3-Qbeta
Date:4/6/2018
[edit]redo PCR of 3127
new primer
3127-HindIII-R
3127-NheI-F2
size:403bp
extension time:30s
3127 subclone
Size:408 bp
Insert:939pJet1.2
Vector:1727 pT7A30
Enzymes:Nde1+HindIII
Miniprep of 944_3166_3127
| # | Lane | Size | Enzymes | Result | Note |
| 944 | 1-4; 5 is 944pminiT2 | 2334bp | Nde1+Xho1 | Size is a little bigger than 2334 bp, need to do sequencing | 944pMiniT2 is from PCR |
| 3166 | 5-9; 10 is 3166pminiT2 | 1234bp | BsrG1+Sal1 | to do sequence | 3166pMiniT2 is from PCR |
| 3127 | 11-14; 14 is 3127pMiniT2 | 402bp | Nde1+HindIII | to do sequence | 3127pMiniT2 is from PCR |
Result:Because used the vector pT7A3 not pET28b-qBeta, so name this a new clone as 997 and redo 3127