986 pT7A3-6His-NLS-Cas9-NLS-6xHis

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PCR of 986

F1:986-F1+986-R1

F2:986-F2+986-R2

Size:4kbp

Extension time:4min

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Date:4/5/2018

[edit]

subclone of 986

Vector:1708

For 986 cloning

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Miniprep of 986

#Ends Coordinates Length (bp) 
1 HindIII-HindIII 1124-3468 2345 
2 HindIII-HindIII 93-1123 1031 
3 HindIII-HindIII 3469-92 893 

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