Yijia LI 4 16 2018
Date:4/19/2018
[edit]Miniprep digestion of 824_984_3122
824:1-5, 5 is sequenced + Vector:1518pET28b
984:6-10, 10 is sequenced + Vector:1987pT7A3
3122:11-15, 15 is sequenced + Vector:1727 pT7A30
Result:suspicious result need to repeat
Miniprep of 931
Enzymes:Nde1+Xho1
Size:2160bp
Result:good, but need to confirm with Nde1+HindIII.Nde1+Xho1.HindIII+Xho1
Date:4/20/2018
[edit]Miniprep of 3122
Enzymes:Nde1+Xho1
Sample:#1-4
Lane #5 is sequenced +
Miniprep of 931_824
| # | Lane | control lane | enzymes | size | note |
| 931--2 | 1-3 | F1--lane10, F2--lane11 |
1-Nde1+Xho1; 2-Nde1+HindIII; 3-HindIII+Xho1 |
||
| 931--3 | 4-6 | F1--lane10, F2--lane11 |
4-Nde1+Xho1; 5-Nde1+HindIII; 6-HindIII+Xho1 |
||
| 931--4 | 7-9 | F1--lane10, F2--lane11 |
7-Nde1+Xho1; 8-Nde1+HindIII; 9-HindIII+Xho1 |
||
| 824 | 12-15 | lane 16 832pMiniT2 | Nde1+Xho1 |
