Yijia LI 5 14 2018
Miniprep digestion of 3122_984_3192_3194
| # | Lane | Enzymes | Size | Note |
| 3122 | 1-4 | Nde1+Xho1 | ||
| 984 | 5-8 | Xba1+Nhe1 | 957bp | Lane 8 maybe good,need to confirm the size |
| 3192 | 9-12 | Kpn1 | need to confirm the size | |
| 3194 | 13-16 | Nhe1+Kpn1 | Maybe vector |
Miniprep digestion of 1000_3200_3166
| # | Lane | Enzymes | Size | Note |
| 1000 | 1-3 | HindIII+Xho1 | 100 | need to confirm the size |
| 3200 | 4-6 | BamH1+ECoR1 | no insert band should be good,redo | |
| 3166 | 7-10 | Sal1+BsrG1 |
1234 | need to confirm the size |
Miniprep digestion of 3191_931_3197
| # | Lane | ENzymes | Size | Note |
| 3191 | 1 | Not good, redo | ||
| 931 | 2,3 | 2--HindIII+Nde1;3--HindIII+Xho1 | both are around 900 bp | seems good, but lysis |
| 3197 | 4,5 | not good, lysis |
Conclusion
[edit]Potentially Good:984,3192,3166