3166 pCMV-HA-VH-mIgG2b
Date:3/15/2018
3166 PCR
Primer:3166F+3166R
Template:3017pCEL0
buffer:Platinum
length:60bp
extension time:15s
Date:3/21/2018
Miniprep of 3166(from PCR)
Restriction enzymes:BsrGI+NdeI
Buffer:Fastdigest
Time:30min
Result:#1,#3,#5 have bands
Screening of 3166 miniprep
1,3,5: BsrGI+SalI
1,3,5:BsrGI
Time:15min
Buffer:Fastdigest
Result:good
size is not right, should be 1.5 kB
Date:2/26/2018
Test of 947_945_3166_3165
| Name | Enzymes | Size (bp) | Lane |
| 947 | BglII | 646 | 1,2,3 |
| BssHII | 1856 | 4,5,6 | |
| 945 | BglII | 628 | 11,12 |
| BssHII | 1838 | 13 | |
| 3165 | SalI+BsrGI | 693 | 8 |
| HindIII+BsrGI | 441 | 10 | |
| 3166 | SalI+BsrGI | 1234 | 9 |
| XbaI | 2049 | 7 | |
| BglII | 3259 | to do |
Date:5/21/2018
[edit]Miniprep digestion of 1000_3200_3166
| # | Lane | Enzymes | Size | Note |
| 1000 | 1-3 | HindIII+Xho1 | 100 | need to confirm the size |
| 3200 | 4-6 | BamH1+ECoR1 | no insert band should be good,redo | |
| 3166 | 7-10 | Sal1+BsrG1 |
1234 | need to confirm the size |
sequencing using mIgG2b-HC-PCR-R GACTCTGGGATGCCTGGTCAA