984 pT7A3-6H-SBP-SUMO-3C
Date:4/2/2018
[edit]subclone of 984
Vector:1987
Insert:982
Enzymes:Xba1-Nhe1
Date:4/4/2018
[edit]miniprep digestion of 984_947_3152
- Name Enzymes Size:
1-4:984 XbaI+NheI 957bp
5 :982(973-1pMiniT) XbaI+NheI
6-9:947 XbaI+NcoI-HF 1344bp
10:947pMiniT XbaI+NcoI-HF
11:947(from PCR cloning) XbaI+NcoI-HF
12-15:3152 BsrGI+NheI 371bp
16: 3162(Vector) BsrGI+NheI
Date:4/5/2018
[edit]Subclone 984
Vector:1987
Insert size:957 bp
subclone of 984
Vector:1987
Insert:982
Size:957 bp
Enzymes:Xba1-Nhe1
Date:5/29/2018
[edit]Subclone and Vector digestion
Miniprep digestion of 984_931
| # | Lane | Enzymes | Size | Note |
| 984 | 2-5 | Xba1+Nhe1 | 957bp | #6,7,,8 seems good |
| 931 | 1,6-8 | HindIII+Xho1 | 986bp | #5 seems good |
samples are mislabled?
not good.sequencing sample: clone #, result: Junk
Date:5/6/2018
[edit]Miniprep of 3191_984_995
