Vector vs Sequencing Primers
| Vector Name | Clone ID | Description | 5' Primer | Primer Lot, Location | 3' Primer | Primer Lot, Location | Note |
| pCMV5 | many | CMV5 promoter | CMV5-seq-F | ||||
| pEPD |
3200 | based on pADL-23c | pADL23C-seq-F CCAGGCTTTACACTTTATGCTTC |
pADL23C-seq-R GTTGAAAGTTGTTTAGCAAAACC |
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| pADL-23c |
3092 |
pADL23C-seq-F |
pADL23C-seq-R |
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| pT7A3 | 1279 |
pT7A3-seq-F, difficult template | T7 R (T7 Term) |
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| pT7A30 | 1727 |
pT7A3-seq-F, difficult template |
T7 R (T7 Term) |
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| pET28b | T7 F | T7 R (T7 Term) |
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| pET30b | T7 F |
T7 R (T7 Term) |
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| pT7A30-6His-pMBP |
947 |
6His-pMBP |
MBP-seq-F |
T7 R (T7 Term) |
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| pT7A30-pMBP |
945 |
pMBP | MBP-seq-F |
T7 R (T7 Term) |
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| pCold-MBP-(3C) |
1611 |
8XHis-MBP-(3C) |
MBP-seq-F GATGAAGCCCTGAAAGACGCGCAG |
T7 R (T7 Term) |
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| pCold-MBP-(E3C) |
1804 |
MBP-(E3C) | MBP-seq-F |
T7 R (T7 Term) |
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| pCold-6H-(TEV)-MBP-(3C) |
1756 |
6XHis-MBP-(E3C) |
MBP-seq-F |
T7 R (T7 Term) |
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| pCold-MBP-(a3C)-MCS-3C |
1807 |
MBP-(a3C)-MCS-3C |
MBP-seq-F |
N/A | |||
| pCold-6His-MCS-(TEV-3C)-MBP |
1798 |
6His-MCS-(TEV-3C)-MBP |
pCold-seq-F |
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| pCold-GST-(3C) |
1629 |
GST-seq-F |
2012_5_21 B11 |
T7 R (T7 Term) |
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| pCold-8XHis-MBP-(3C)-MCS |
1659 |
8His-MBP-(3C) | MBP-seq-F |
T7 R (T7 Term) |
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| pCold-7His-GST-(E) |
1612 |
7His-GST-(E) | GST-seq-F |
T7 R (T7 Term) |
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| pCold-6His-GST-(E)-(3C) |
1624 |
6His-GST-(E)-(3C) |
GST-seq-F |
T7 R (T7 Term) |
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| pET28-cold |
v19 |
pCold-seq-F |
pCold-seq-R |
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| pCold-6H | 1755 |
pCold-seq-F AGCACGCCATATCGCCGAAAG |
3_11_2013 B05 |
pCold-seq-R GGCAGGGATCTTAGATTCTG or T7 R (T7 Term) |
3_11_2013 B06 |
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| pCold-TF-(E3C)-3C |
1729 |
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| |
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| pEF1-HTLV-1IFH-HA-VH-mIgG2b |
3165 |
hEF1-HTLV-seq-F CTCCCTTGGAGCCTACCTAG EF1-HTLV-seq-F CTGCTTGCTCAACTCTACGTC |
2012_5_15 A06 , 9/14/17 |
pFuse-seq-R GCATTCTAGTTGTGGTTTGTCC |
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| pCMV-HA-VH-mIgG2b |
3166 |
CMV-F | (Genscript) | ||||