Yijia LI 6 5 2018

Miniprep of 3122_3210

Miniprep of 3191_984_995

Miniprep of 3196_2503

Date:6/6/2018

PCR 3197

PCR 3208_3218

PCR of 998_1000_3194

PCR of 994

PCR of 995

PCR of 3218 Fragment 2

PCR of 3206

1st PCR of 3208

2nd PCR of 3208

PCR of 3204

Date:6/7/2018

2nd PCR o 3206

homemade phusion

2nd PCR of 3218

homemade phusion

Dat3:6/8/2018

PCR of 3232

Vector digestion of 3232

Miniprep of 3191

Miniprep of 3122

Vector digestion of 994_995_3191_3197_3218_3206_998_1000

2503 subclone

931-1 subclone

2509 subclone

3210 subclone

3196 subclone

3233 subclone_3194 vector digestion

3199 subclone