1916 pT7A3E-HC2-M-SssI
Cloning of 1916 pT7A3E-HC2-M-SssI
[edit]Purpose: Cloning of1916 pT7A3E-HC2-M-SssI==
Date: 2014-9-15---
Notebook:0
Page:
Clone ID:1916
Experimenters: shanyi
Results
[edit]CLONING
[edit]gene synthesis
join two fragments by PCR
Digest with Nde1 + Xho1
Colony Screening
[edit]Date: 2014_9
Make miniprep, use Nde1 + Xho1 to find positive clone
Result intepretation/Summary
[edit]Conclusion
[edit]====
1916 in pMiniT2
6/4/2018, gene synthesis
digest with EcoR1, submit #3 for sequencing
1916 amplified by combining two fragments: one from above, another from IDT gene fragment
miniprep digest with EcoR1:
6/22/18: #2 sequencing : missing missing GTT between tow fragments