3234 pCMV5-IL2-PD-1-(3c)-Fc-WPRE-OriP
Cloning of
[edit]Purpose:
Date: 2018-6-22
Notebook:
Page:
Clone ID:3237
Experimenters: Paul
Results
[edit]PCR
[edit]Date: Date: 6/21/2018
By: Paul
template:
primers:
Gel:
http://www.excellgen.net/eln/images/5/52/3237_3232_3234_PCR.JPG
Cloning
By: Paul
Vector:
Ligation: 30 min with T4 DNA ligase
Competent cells: NEBTurbo
Colony PCR Screening
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Restriction Digestion
Date: 6/23/2018
By: Paul
Sequencing