3232 pCMV5-Avi-6H-BirA

Dat3:6/8/2018

PCR of 3232

Vector digestion of 3232

Cloning of

Purpose:  

Date: 2018-6-22

Notebook: 

Page:

Clone ID:3237

Experimenters: Paul 

Results

PCR

Date: Date: 6/21/2018

By: Paul

template:

primers:

Gel:

http://www.excellgen.net/eln/images/5/52/3237_3232_3234_PCR.JPG

Cloning

By: Paul

Vector:  

Ligation: 30 min with T4 DNA ligase

Competent cells: NEBTurbo

Colony PCR Screening 

Restriction Digestion

Date:  6/23/19, by Paul

http://www.excellgen.net/eln/images/2/2a/3237_3232_3234_minipre_digestion.JPG

Sequencing, #1 is good