2503 pCold-GST-VVTopo
Subclone and Vector digestion
Miniprep digestion of 2503_3122_3197_3191
| # | lane | Enzymes | Size | Note |
| 2503 | 1-4 | Nde1+Xho1 | 951bp | #1,2 seems good |
| 3122 | 5-8 | Nde1+Xho1 | 1743bp | #5,6,8 seems good |
| 3197 | 9-12 | BamH1+Nco1 | 1 band | may not good |
| 3191 | 13-16 | BamH1+Nco1 | 722bp | #14,15,16 seems good |
sequencing sample #: result: no priming, possible reason: ??
need to do: digest with EcoR1+ Xho1, Pst1+ Xho1, and Nde1 + Xho1 5/31/2018: Sequencing sample #: result: vector
Date:6/5/2018==
2503 subclone
Miniprep of 3196_2503
2503 subclone
Miniprep of 2503_3210_931-1
digestion using Nhe1 + Xho1
vector is 3161 pCold-GST--Myc-HA-Flag-V5
Subclone from 832 pMiniT-VVTopo