345 pET30b-Ent

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Cloning of  by Gene Synthesis

Purpose: to clone

Date: 2011-9-26

Notebook: luyingying

Page:0

Clone ID:345

Experimenters: lu yingying ,
Results

345-enterokinase-PCR.JPG

All bands show PCR product of Enterokinase

template:enterokinase gene synthesis ,enterokinase1 to enterokinase 22

primerF: enterokinase1  primerR: enterokinase22

345enterokinase digest9-26-2011.JPG

band show enterokinase digest result:Nco1 +Xho1

345-PET30b digest-9-26-2011.JPG

All bands show PET30b digest result:Nco1 +Xho1


bands show 6 different clones digest result:Nco1 +Xho1


Gene Synthesis

Colony Screening

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