3204 pCMV5-OX40-Avi-6H-BirA
Cloning of 3204 pCMV5-OX40-Avi-6H-BirA
[edit]Purpose: Cloning of 3204 pCMV5-OX40-Avi-6H-BirA
Date: 06, 20, 2018
Notebook:
Page:
Clone ID: 3204
Experimenters: yijia
Results
[edit]Cloning
[edit]PCR from 3203 pANT7_cGST-TNFRSF4
PCR of 3204
Vector digestion
Vector is 1393
Vector digestion_3204cubclone_931-1 subclone
Colony screen
[edit]Miniprep of 3215_3204
| # | Lane | Vector | Clone # Name | Method | Enzymes | Size |
| 3215 | 1-5 | 3192 | 1-5 | PCR | Nhe1+Kpn1 | 285 |
| 3204 | 6-10 | 1393 | 1-5 | PCR | Nhe1+Kpn1 | 564 |
3204 digestion
Enzymes:Age1+Knp1
Result:#3,#4,#5, are good, #1,#2 might be vector
Miniprep digestion _3204_3215_941
