Yijia LI 6 18 2018

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Date:6/18/2018

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PCR of 994

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PCR of 995

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Miniprep of 2509

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PCR of 3215

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Vector digestion

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Date:6/20/2018

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Miniprep of 3215_3204

# Lane Vector Clone # Name Method Enzymes Size
3215 1-5 3192 1-5 PCR Nhe1+Kpn1 285
3204 6-10 1393 1-5 PCR Nhe1+Kpn1 564

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3204 digestion

Enzymes:Age1+Kpn1

Result:#3,#4,#5, are good

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Date:6/22/2018

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1st PCR fo 3218

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2nd PCR of 3218

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2509subclone

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Vector digestion_1000_3206_3197

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1st PCR of 3206

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2nn PCR of 3206

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3197 PCR

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Vector digestion&3225 subclone

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PCR of 1000

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