3231 pEPD-DP47-DPK22

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Cloning of

Purpose:

Date: 2018-6-22

Notebook:

Page:

Clone ID:3237

Experimenters: Paul

Results

PCR

Date: Date: 6/21/2018

By: Paul

template:

primers:

Gel:

Cloning

By: Paul

Vector:

Ligation: 30 min with T4 DNA ligase

Competent cells: NEBTurbo

Colony PCR Screening

~

Restriction Digestion

Date: 6/~/2018

By: Paul

http://www.excellgen.net/eln/images/8/8c/3208_3231_3230_digestion.JPG

Sequencing

new 3231, digest with BamH1 -Xho1

7/2/18

clone #2: sequencing is good

Result intepretation/Summary

Conclusion

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Cloning