Yijia LI 7 2 2018
Date:7/2/2018
[edit]1st PCR of 3218
Miniprep of 129_994_1000_3206
| # | Vector | Lane | Enzymes | Size | Clone# | Note |
| 129 | 1801 | 1-4 | Nde1+HindIII | 2433 | 2,4 look good | 129-2;129-4 |
| 994 | 3225 | 5-8 | BamH1+Xho1 | 609 | 5,6,look good | Vecotor doesn't have BamH1 Site,994-1;994-2 |
| 1000 | 1708 | 9-12 | HIndIII+Xho1 | 118 | need to do PCR screening | |
| 3206 | 3165 | 13-16 | HIndIII+BamH1 | 1460 | need to do PCR screening |
Date:7/6/2018
[edit]