3197 pT7A3-GST-BCMA
Vector digestion
PCR of 3197
Miniprep digestion of 2503_3122_3197_3191
| # | lane | Enzymes | Size | Note |
| 2503 | 1-4 | Nde1+Xho1 | 951bp | #1,2 seems good |
| 3122 | 5-8 | Nde1+Xho1 | 1743bp | #5,6,8 seems good |
| 3197 | 9-12 | BamH1+Nco1 | 1 band | may not good |
| 3191 | 13-16 | BamH1+Nco1 | 722bp | #14,15,16 seems good |
not good
Cloning of 3197 pT7A3-GST-BCMA
[edit]Purpose: Cloning of 3197 pT7A3-GST-BCMA
Date: 06, 22, 2018
Notebook: 008840
Page:008956
Clone ID: 3197
Experimenters: yijia
Cloning
[edit]Vector digestion_1000_3206_3197
Vector is 1851
Results
[edit]Colony screen
[edit]PCR screening of 3197
Miniprep digestion
sequencing result: all are empty vector
Redo
[edit]PCR
[edit]6/27/2018
by yijia
Miniprep diegstion
[edit]Enzymes:BamH1+BgIII
Vector is 3225
| # | Lane | Enzymes | Size | Note |
| 3197 | 1-4 | BamH1+BglII | 884 | Maybe good. 3197-1--3197-4 |
redo 3197 PCR
3197 miniprep digestion
3197 pcr SCREENING
| # | Lane | 72oC annealing | Note | |
| 3197 | 1-8 | 15s | #3,#4,#6,#7 | |
| control | v1851;v3225; ----- no template | 15s |
