Cloning report
| ID | Status | Name | Progress |
Note |
| 812 |
pET30b-phoA |
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| 931 | PAK16D ligase |
pJet1.2 fragment 2 is good fragment 1 is also good need to do digestions seqeucning result: junk, E coli chromesome DNA |
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| 988 |
987 pCMVS-LAMP-1_CD133 |
- | ||
| 998 |
pT7A3-6His-NLS-dCas9-NLS-6xHis |
6/15/18:bands on gel seems good but use wrong template because of data base,right one should be 978 not 979 |
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| 999 |
pT7A30-SssI |
based on 1916 gene synthesis | ||
| 1000 | pT7A3-6His-NLS-FnCpf1-NLS-6xHis |
3' is good | ||
| 3218 |
pT7A30-ULP1 |
PRC from 2209 ZM552-ULP1 | pickup | |
| 3204 |
pCMV5-OX40-Avi-6H-BirA |
from 3203 pANT7_cGST-TNFRSF4 |
subclone fnished,vector3237 | |
| 3195 |
pEF1-HTLV-LAMP-1-BCMA |
doing 3206 vector now | ||
| 3225 | pT7A3-GST-MICA |
subclone fnished | ||
| 3215 | pCMV5-MICA-a-Avi-6H-BirA |
subclone finished | ||
| 3208 |
pEPD-FW |
sequencing finished | ||
| 941 | pT7A3-pMBP-T7-Endo-1 |
pickup on 6/29/2018 | ||
| 3234 | pCMV5-IL2-PD-1-(3c)-Fc-WPRE-OriP |
sequence finished | ||
| 3235 |
pCMV5-IL2-MICA-(3c)-Fc-WPRE-OriP |
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| 3234 |
pCMV5-IL2-PD-1-(3c)-Fc-WPRE-OriP |
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| 3233 |
pCMV5-IL2-CD47-(3c)-Fc-WPRE-OriP |
good, need to make glycerol stock | ||
| 3232 |
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