3215 pCMV5-MICA-a-Avi-6H-BirA

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Cloning of 3215 pCMV5-MICA-a-Avi-6H-BirA

Purpose: Cloning of 3215 pCMV5-MICA-a-Avi-6H-BirA

Date: 06, 20, 2018

Notebook:

Page:

Clone ID: 3215

Experimenters: yijia

Results

Cloning

PCR from 3213 pANT7_cGST-MICA

PCR of 3215

Vector digestion

Vector is 3192

3215subclone

Colony screen

Miniprep of 3215_3204

#	Lane	Vector	Clone # Name	Method	Enzymes	Size
3215	1-5	3192	1-5	PCR	Nhe1+Kpn1	285
3204	6-10	1393	1-5	PCR	Nhe1+Kpn1	564

Miniprep digestion _3204_3215_941

Vector is 3237

Result intepretation/Summary

Conclusion

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Cloning