3218 pT7A30-ULP1
Cloning of 3218 pT7A30-ULP1
[edit]Purpose: Cloning of 3218 pT7A30-ULP1
Date: 06, 22, 2018
Notebook: 008840
Page:008956
Clone ID: 3218
Experimenters: yijia
Results
[edit]Cloning
[edit]PCR from 2209 ZM552-ULP1
1st PCR fo 3218
2nd PCR of 3218
Vector digestion
Vector digestion&3225 subclone
Colony screen
[edit]Miniprep digestion of 3218
redo
[edit]2nd PCR 3218
Miniprep digestion of 3218
Miniprep digestion of 3218_998
| # | lane | Enzymes | Size | Result |
| 3218 | 1-8 | HindIII | 617bp | no good |
2nd Redo
[edit]1st PCR of 3218
Template:2209
F1:3218-F3+3218-R4
F2:3218-F4+3218-R1
5X enhancer, homemade phusion, 5x thermo phusion HF buffer
2nd PCR of 3218
Template:1st PCR above
F1:3218-F1+3218-R1
F2:3218-F2+3218-R2
5X enhancer, homemade phusion, 5x thermo phusion HF buffer
Result intepretation/Summary
[edit]3218 miniprep digestion
3218--#9 need to confirm
3218-#8,,same thing as #9 above,PCR screening
3218-8 digetsion
