3206 pEF1-HTLV-LAMP-1
Cloning of 3206 pEF1-HTLV-LAMP-1
[edit]Purpose: Cloning of 3206 pEF1-HTLV-LAMP-1
Date: 06, 22, 2018
Notebook: 008840
Page:008956
Clone ID: 3206
Experimenters: yijia
Results
[edit]Cloning
[edit]PCR from 987 pANT7_cGST-LAMP1
Vector digestion_1000_3206_3197
Vector is 3165
1st PCR of 3206
2nn PCR of 3206
Colony screen
[edit]Miniprep diegstion of 3206
need to do PCR screening
Redo
[edit]3206 1st PCR
Template:987
Fragment 1: 3206-F1 + 988-R1
Fragment 2: 988-F3 + 3206-R3
5X enhancer,homemade phusion, 5X thermo phusion HF buffer
3206 2nd PCR
Template:1st PCR above
Fragment 1: 3206-F1 + 3206-R4
Fragment 2: 3206-F2 + 3206-R3
5X enhancer,homemade phusion, 5X thermo phusion HF buffer
Result intepretation/Summary
[edit]3206 PCR Screening
