1000 pT7A3-6His-NLS-FnCpf1-NLS-6xHis
Cloning of 1000 pT7A3-6His-NLS-FnCpf1-NLS-6xHis1
[edit]Purpose: Cloning of 1000 pT7A3-6His-NLS-FnCpf1-NLS-6xHis
Date: 06, 22, 2018
Notebook: 008840
Page:008956
Clone ID: 1000
Experimenters: yijia
Results
[edit]Cloning
[edit]PCR from 980 pET28amp-6His-MBP-TEV-FnCpf1
PCR of 1000
Vector digestion_1000_3206_3197
Redo
1000 PCR
Colony screen
[edit]Miniprep of 129_994_1000_3206
| # | Vector | Lane | Enzymes | Size | Clone# | Note |
| 129 | 1801 | 1-4 | Nde1+HindIII | 2433 | 2,4 look good | 129-2;129-4 |
| 994 | 3225 | 5-8 | BamH1+Xho1 | 609 | 5,6,look good | Vecotor doesn't have BamH1 Site,994-1;994-2 |
| 1000 | 1708 | 9-12 | HIndIII+Xho1 | 118 | Lane11,Lane12,Lane13 are good, do sequencing | |
| 3206 | 3165 | 13-16 | HIndIII+BamH1 | 1460 | need to do PCR screening |
