Rabbit Antibody Cloning

Signal Peptide

atggagactgggctgcgctggcttctcctggtcgctgtgctcaaaggtgtccagtgt
 M  E  T  G  L  R  W  L  L  L  V  A  V  L  K  G  V  Q  C 

VH

cagtccctggaggagtccgggggtcgcctggtcaagcctggaggaaccctgacactcacctgc
 Q  S  L  E  E  S  G  G  R  L  V  K  P  G  G  T  L  T  L  T  C 
acagtctctggaatcgacctcagtagctcgggagtgagctgggtccgccaggctccaggg
 T  V  S  G  I  D  L  S  S  S  G  V  S  W  V  R  Q  A  P  G 
aaggggctggaatacatcggatacattagtagcagtggtagcacatactacgcgagctgg
 K  G  L  E  Y  I  G  Y  I  S  S  S  G  S  T  Y  Y  A  S  W 
gcgaaaggccgattcaccatctccaaaacctcgtcgaccacggtggagctgaaagtgacc
 A  K  G  R  F  T  I  S  K  T  S  S  T  T  V  E  L  K  V  T 
agtctgacaactgaggacacggccacctatttctgtagtagaaatggtctaggggccttg
 S  L  T  T  E  D  T  A  T  Y  F  C  S  R  N  G  L  G  A  L 
tggggccaaggcaccctggtcaccgtt
 W  G  Q  G  T  L  V  T  V 

IgG Constant Heavy (allele 3/2)

tcgagtgggcaacctaaggctccatcag
 S  S  G  Q  P  K  A  P  S   ....

TCGAGTGGGCAACCTAAGGCTCCATCAGTCTTCCCACTGGCCCCCTGCTGCGGGGACACACCCAGCTCCACGGTGACCCTGGGCTGCCTGGTCAAAGGCTACCTCCCGGAGCCAGTGACCGTGACCTGGAACTCGGGCACCCTCACCAATGGGGTACGCACCTTCCCGTCCGTCCGGCAGTCCTCAGGCCTCTACTCGCTGAGCAGCGTGGTGAGCGTGACCTCAAGCAGCCAGCCCGTCACCTGCAACGTGGCCCACCCAGCCACCAACACCAAAGTGGACAAGACCGTTGCG CCCTCGACATGCAGCAAGCCCACGTGCCCACCCCCTGAACTCCTGGGGGGACCGTCTGTCTTCATCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCACGCACCCCCGAGGTCACATGCGTGGTGGTGGACGTGAGCCAGGATGACCCCGAGGTGCAGTTCACATGGTACATAAACAACGAGCAGGTGCGCACCGCCCGGCCGCCGCTACGGGAGCAGCAGTTCAACAGCACGATCCGCGTGGTCAGCACCCTCCCCATCGCGCACCAGGACTGGCTGAGGGGCAAGGAGTTCAAGTGCAAAGTCCACAACAAGGCACTCCCGGCCCCCATCGAGAAAACCATCTCCAAAGCCAGAGGGCAGCCCCTGGAGCCGAAGGTCTACACCATGGGCCCTCCCCGGGAGGAGCTGAGCAGCAGGTCGGTCAGCCTGACCTGCATGATCAACGGCTTCTACCCTTCCGACATCTCGGTGGAGTGGGAGAAGAACGGGAAGGCAGAGGACAACTACAAGACCACGCCGGCCGTGCTGGACAGCGACGGCTCCTACTTCCTCTACAGCAAGCTCTCAGTGCCCACGAGTGAGTGGCAGCGGGGCGACGTCTTCACCTGCTCCGTGATGCACGAGGCCTTGCACAACCACTACACGCAGAAGTCCATCTCCCGCTCTCCGGGTAAATGAGCGCTCCTAGGTCTAGACCTAG

SSGQPKAPSVFPLAPCCGDTPSSTVTLGCLVKGYLPEPVTVTWNSGTLTNGVRTFPSVRQSSGLYSLSSVVSVTSSSQPVTCNVAHPATNTKVDKTVAPSTCSKPTCPPPELLGGPSVFIFPPKPKDTLMISRTPEVTCVVVDVSQDDPEVQFTWYINNEQVRTARPPLREQQFNSTIRVVSTLPIAHQDWLRGKEFKCKVHNKALPAPIEKTISKARGQPLEPKVYTMGPPREELSSRSVSLTCMINGFYPSDISVEWEKNGKAEDNYKTTPAVLDSDGSYFLYSKLSVPTSEWQRGDVFTCSVMHEALHNHYTQKSISRSPGK

Full length

atgagctgggtccgacaggctccagggaaggagctggagtggatcggatacattagttat
 M  S  W  V  R  Q  A  P  G  K  E  L  E  W  I  G  Y  I  S  Y 
ggtggtagtgcatactacgcgagctgggcgaaaagccgatccaccatcaccagaaacacc
 G  G  S  A  Y  Y  A  S  W  A  K  S  R  S  T  I  T  R  N  T 
aacgagaacacggtgactctgaaaatgaccagtctgacagccgcggacacggccacctat
 N  E  N  T  V  T  L  K  M  T  S  L  T  A  A  D  T  A  T  Y 
ttctgtgcgagacattggggcatctggggcccaggcaccctggtcaccgtc
 F  C  A  R  H  W  G  I  W  G  P  G  T  L  V  T  V  

tcctcagggcaacctaaggctccatcagtcttcccactggccccctgctgcggggacaca
 S  S  G  Q  P  K  A  P  S  V  F  P  L  A  P  C  C  G  D  T 
cccagctccacggtgaccctgggctgcctggtcaaaggctacctcccggagccagtgacc
 P  S  S  T  V  T  L  G  C  L  V  K  G  Y  L  P  E  P  V  T 
gtgacctggaactcgggcaccctcaccaatggggtacgcaccttcccgtccgtccggcag
 V  T  W  N  S  G  T  L  T  N  G  V  R  T  F  P  S  V  R  Q 
tcctcaggcctctactcgctgagcagcgtggtgagcgtgacctcaagcagccagcccgtc
 S  S  G  L  Y  S  L  S  S  V  V  S  V  T  S  S  S  Q  P  V 
acctgcaacgtggcccacccagccaccaacaccaaagtggacaagaccgttgcaccctcg
 T  C  N  V  A  H  P  A  T  N  T  K  V  D  K  T  V  A  P  S 
acatgcagcaagcccacgtgcccaccccctgaactcctggggggaccgtctgtcttcatc
 T  C  S  K  P  T  C  P  P  P  E  L  L  G  G  P  S  V  F  I 
ttccccccaaaacccaaggacaccctcatgatctcacgcacccccgaggtcacatgcgtg
 F  P  P  K  P  K  D  T  L  M  I  S  R  T  P  E  V  T  C  V 
gtggtggacgtgagccaggatgaccccgaggtgcagttcacatggtacataaacaacgag
 V  V  D  V  S  Q  D  D  P  E  V  Q  F  T  W  Y  I  N  N  E 
caggtgcgcaccgcccggccgccgctacgggagcagcagttcaacagcacgatccgcgtg
 Q  V  R  T  A  R  P  P  L  R  E  Q  Q  F  N  S  T  I  R  V 
gtcagcaccctccccatcacgcaccaggactggctgaggggcaaggagttcaagtgcaaa
 V  S  T  L  P  I  T  H  Q  D  W  L  R  G  K  E  F  K  C  K 
gtccacaacaaggcactcccggcccccatcgagaaaaccatctccaaagccagagggcag
 V  H  N  K  A  L  P  A  P  I  E  K  T  I  S  K  A  R  G  Q 
cccctggagccgaaggtctacaccatgggccctccccgggaggagctgagcagcaggtcg
 P  L  E  P  K  V  Y  T  M  G  P  P  R  E  E  L  S  S  R  S 
gtcagcctgacctgcatgatcaacggcttctacccttccgacatctcggtggagtgggag
 V  S  L  T  C  M  I  N  G  F  Y  P  S  D  I  S  V  E  W  E 
aagaacgggaaggcagaggacaactacaagaccacgccggccgtgctggacagcgacggc
 K  N  G  K  A  E  D  N  Y  K  T  T  P  A  V  L  D  S  D  G 
tcctacttcctctacaacaagctctcagtgcccacgagtgagtggcagcggggcgacgtc
 S  Y  F  L  Y  N  K  L  S  V  P  T  S  E  W  Q  R  G  D  V 
ttcacctgctccgtgatgcacgaggccttgcacaaccactacacgcagaagtccatctcc
 F  T  C  S  V  M  H  E  A  L  H  N  H  Y  T  Q  K  S  I  S 
cgctctccgggtaaatga
 R  S  P  G  K  -  
<pre>


== Light Chain Kappa1 ==

GATCCAGTTGCACCTACTGTCCTCATCTTCCCACCAGCTGCTGATCAGGTGGCAACTGGAACAGTCACCATCGTGTGTGTGGCGAATAAATACTTTCCCGATGTCACCGTCACCTGGGAGGTGGATGGCACCACCCAAACAACTGGCATCGAGAACAGTAAAACACCGCAGAATTCTGCAGATTGTACCTACAACCTCAGCAGCACTCTGACACTGACCAGCACACAGTACAACAGCCACAAAGAGTACACCTGCAAGGTGACCCAGGGCACGACCTCAGTCGTCCAGAGCTTCAACAGGGGTGACTGCTAG

DPVAPTVLIFPPAADQVATGTVTIVCVANKYFPDVTVTWEVDGTTQTTGIENSKTPQNSADCTYNLSSTLTLTSTQYNSHKEYTCKVTQGTTSVVQSFNRGDC-

<pre>
Aligment of some VL domains
5       mdtraptqllgllllwlpgatfavvltqtpssvsaavggsvtiscqssqslynnnrlawy
4       ----------------------alvmtqtpssvsaavggtvtincqasqsvgnnnnlvwy
6       ----------------------dvvmtqtpspvsaavggtvsincqasqsvyknnylswy
8       ---------------------mdvvmtqtpspvsaavgstvtincqasqsvynnhylswy
1       ----------------------divmtqtpasvsaavggtvtincqasetis--nylawy
2       ----------------lpgarcaydmtqtpasvevavggtvtikcqasqsis--tylswy
7       ---------------------aevvmtqapssveaavggtvtikcqasrsin--nylswy
9       mdmraptqllgllllwlpgarcdvvmtqtpasveapvggtvtikcqasqsvs--gycswy
3       ---------------------mdvvmtqtpasveaavggtvtikcqasqniy--snlawy
10      ----------------------dvvmtqtpasvsepvggtvtikcqasqsis--sylawy
                                 :**:*: *.  **.:*:*.**:*..:       **

5       qqkagqppklliykastlesgvpsrfkgsgagtqftltisdvvcddaatyycag-yksn-
4       qqkpgqppklliyrasnletgvpsrfkgsgsgteftltisdlecddaatyycagaysg--
6       qqkpgqrpklliygasnlesgvpsrfkgsgsgteftltisdvqcddaatyyclgeygsr-
8       qqkpgqppkrliyaastlesgvssrfkgsgsgtqftltisdvqcddaatyyclgsydcs-
1       qqkpgqppklliykastlasgvssrfkgsgsgteytltisgvqcddaatyycqqgysisd
2       qqkpgqrpklliyrastlasgvssrfkgsgsgteftltisgvecadaatyycqqgwsssn
7       rqkpgqppklliyeasklasgvpsrfsgsgsgteftltisdlecadaatyycqglyfvsh
9       qqkpgqppklliyrastlesgvpsrfsgsgsgtdftltisdlecadaatyycqsnynsgs
3       qqkpgqrpklliykastlasgvssrfkgsgsgteftltisdlecadaatyycqctwydn-
10      qqkpgqrprlliyetstlasgvpsrfkgsgsgtdftltisdlecadaatyycqstyenp-
        :** ** *: *** :*.* :** ***.***:**::*****.: * *******   :    

5       sddgtafgggtevvvkgdPVAPT
4       --nvihfgggtevvvkgdPVAPT
6       sd--ngfgggtevvvkgdPVAPT
8       sadcfafgggtevvvkgdPVAPT
1       --idnsfgggtevvvkgdPVAPT
2       --venvfgggtevvvkgdPVAPT
7       ssygsafgggtevvvkgdPVAPT
9       sssaaafgggtevvvkgdPVAPT
3       --tyvafgggtevvvkgdPVAPT
10      --tyvsfgggtevgvkgdPVAPT
              ******* *********

Light Chain Kappa2

GATCCAGTTGCGCCTTCTGTCCTCCTCTTCCCACCATCTAAGGAGGAGCTGACAACTGGAACAGCCACCATCGTGTGCGTGGCGAATAAATTCTATCCCAGTGACATCACCGTCACCTGGAAGGTGGATGGCACCACCCAACAGAGCGGCATCGAGAACAGTAAAACACCGCAGAGCCCCGAAGACAATACCTACAGCCTGAGCAGCACTCTGTCACTGACCAGCGCACAGTACAACAGCCACAGCGTGTACACCTGCGAGGTGGTCCAAGGCTCAGCCTCACCGATCGTCCAGAGCTTCAATAGGGGTGACTGCTAG

DPVAPSVLLFPPSKEELTTGTATIVCVANKFYPSDITVTWKVDGTTQQSGIENSKTPQSPEDNTYSLSSTLSLTSAQYNSHSVYTCEVVQGSASPIVQSFNRGDC</pre>