Condition:1mM IPTG, 4oC,O/N
| Lane
|
Sample
|
Name
|
MW
|
Note
|
| M1
|
SeeBlue
|
4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191
|
|
|
| 1
|
993(S)
|
pCold-GST-M13-Gene8
|
31.3
|
y?
|
| 2
|
992(S)
|
pCold-GST-V5
|
30.9
|
y?
|
| 3
|
991(S)
|
pCold-GST-Flag
|
30.5
|
Y
|
| 4
|
990(S)
|
pCold-GST-HA
|
30.6
|
Y
|
| 5
|
989(S)
|
pCold-GST-Myc
|
30.7
|
Y
|
| M2
|
homemade protein standard
|
|
|
|
| 6
|
993(IB)
|
|
|
|
| 7
|
992(IB)
|
|
|
|
| 8
|
991(IB)
|
|
|
|
| 9
|
990(IB)
|
|
|
|
| 10
|
989(IB)
|
|
|
|
| Lane
|
Sample
|
Name
|
Type
|
MW
|
Note
|
| M1
|
seeblue
|
4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191
|
|
|
|
| 1
|
947
|
pT7A30-6His-pMBP
|
S
|
46.6
|
Y
|
| 2
|
986
|
pT7A3-6His-NLS-Cas9-NLS-6xHis
|
S
|
164
|
Y
|
| 3
|
945
|
pT7A30-pMBP
|
S
|
48.3
|
Y
|
| M2
|
|
|
|
|
|
| 4
|
947
|
|
IB
|
|
|
| 5
|
986
|
|
IB
|
|
|
| 6
|
945
|
|
IB
|
|
|