380-pCMVK-IL2-Fc
SubCloning of CFP from pet28b-cfp to the pAAV-MCS (254)
[edit]Purpose: SubCloning of CFP from pet28b-cfp to the pAAV-MCS (254).
Date: 2011-8-3 to 2011-8-10
Notebook:
Page:
Clone ID:
Experimenters: Robert, Mindy, Yolanda
Results
[edit]Subcloning
[edit]Date: 2011-8-4
Digest (clone #) with
Digest (clone #) with
cut out insert and vector, ligate and transform
Colony Screening
[edit]Date: 2011-8-10
Make miniprep and digest with BamH1 + Xho1
Lane 1: 1 kb ladder
Lanes 2-6: pAAV-mkate2
Result intepretation/Summary
[edit]- pAAV-mkate2 clones
Conclusion
[edit]Restriction digestion
Shows
Cloning of pCMVK-IL2-Fc
[edit]Purpose: Cloning of intfc to the pCMVK-IRES-TagGFP(382)
Date: 2011-10-03
Notebook:
Page:
Clone ID:
Experimenters: lu yingying, wangxiaojing
Result:
enzyme digest result:Sal1+ HindIII
band 1 shows pCMVK-TagGFP vector
band2,3 show intFc
digest detect:Sal1+HindIII
6 different clones