136 1708 212 pL6H-Tat-Cre

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Subcloning:

For 134: vector 212 and  insert 1707: Nco1 + Xho1, wrong, should use Nde1 + Not1

For 136: Vector 2038 and insert  129, Nco1 - Xho1 


RTENOTITLE

Miniprep digestion

RTENOTITLE

Redo

10/7/2018

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Lane ID Name Enzyme
1 129
Nde1-Not1
2 2038
Nde1-Not1
3 1708
Nco1-Xho1
4 212
Nco1-Xho1
5 3257
EcoR1-Nhe1
6 3260
EcoR1-Nhe1
7 3261
EcoR1-Nhe1
8 3259
EcoR1-Nhe1
9 3258
EcoR1-Nhe1
10 3262
EcoR1-Nhe1

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