Protein Expression Clone Screening 2018
Protein Expression Clone Screening
| Clone ID | Protein Name | Host | Condition | Pellet after lysis | Expression level | Solubility | Gel image | Date | By |
| 1000 | FnCpf1 | Nico21(DE3)plysN |
0.5mM IPTG, induce 4h, 37oC, OD value is around 0.8. |
sticky | Y | Protein_QC_8_10_2018 | 8/10 | yijia | |
| 942 | MBP-VVN | Nico21(DE3)plysN |
0.5mM IPTG, induce 4h 37oC, OD value is around 0.8. |
sticky | Y | Protein_QC_8_10_2018 |
8/10 |
yijia | |
| 938 | PNGase F |
Nico21(DE3)plysN |
0.5mM IPTG, induce 4h 37oC, OD value is around 0.8. |
sticky | ? | Protein_QC_8_10_2018 |
8/10 |
yijia | |
| 939 | Tte-ExoIII |
Nico21(DE3)plysN |
0.5mM IPTG, induce 4h 37oC, OD value is around 0.8. |
sticky | Y | Protein_QC_8_10_2018 |
8/10 |
yijia | |
| 941 | MBP-T7-Endo |
Nico21(DE3)plysN |
0.5mM IPTG, induce 4h 37oC, OD value is around 0.8. |
sticky | ? | Protein_QC_8_10_2018 |
8/10 |
yijia | |
| 3197 | GST-BCMA |
Nico21(DE3)plysN |
0.5mM IPTG, induce 4h 37oC, OD value is around 0.8. |
sticky | Y | 8/10 |
yijia | ||
| 994 | GST-CD133-EC2 |
Nico21(DE3)plysN |
0.5mM IPTG, induce 4 hours at 37oC, OD value is around 0.8. |
sticky | 8/10 |
yijia | |||
| 995 | GST-CD133-EC3 |
Nico21(DE3)plysN |
0.5mM IPTG, induce 4 hours at 37oC, OD value is around 0.8. |
not sticky | 8/10 |
yijia | |||
| 3225 | GST-MICA |
Nico21(DE3)plysN |
0.5mM IPTG, induce 4 h37oC, OD value is around 0.8. |
not sticky | Y | 8/10 |
yijia | ||
| 3210 | Sumo-Ov-ASP1-BCMA |
Nico21(DE3)plysN |
0.5mM IPTG, induce 4 h 37oC, OD value is around 0.8. |
sticky | Y | 8/10 |
yijia | ||
| 3218 | ULP1 |
Nico21(DE3)plysN |
0.25mM IPTG,RT,o/n | not sticky | ? | Protein QC 8 14 2018 |
8/14 | yijia | |
| 3191 | GST-Avitag |
Nico21(DE3)plysN |
0.25mM IPTG,RT,o/n |
not sticky | Y | Protein QC 8 14 2018 |
8/14 | yijia | |
| 824 | VVTopo |
Nico21(DE3)plysN |
0.25mM IPTG,RT,o/n |
sticky | Y | Protein QC 8 14 2018 |
8/14 | yijia | |
| 999 | SssI |
Nico21(DE3)plysN |
0.25mM IPTG,RT,o/n |
sticky | N | Protein QC 8 14 2018 |
8/14 | yijia | |
| 944 | pfu-exo- |
Nico21(DE3)plysN |
0.25mM IPTG,RT,o/n |
sticky |
Y |
Protein QC 8 14 2018 |
8/14 | yijia | |
| 993 | Nico21(DE3)plysN |
1mM IPTG,4oC,o/n |
loose | Protein QC 4 23 18 |
4/23 | yijia | |||
| 992 | Nico21(DE3)plysN |
1mM IPTG,4oC,o/n |
loose | Protein QC 4 23 18 |
4/23 | yijia | |||
| 991 | Nico21(DE3)plysN |
1mM IPTG,4oC,o/n |
loose | Protein QC 4 23 18 |
4/23 | yijia | |||
| 990 | Nico21(DE3)plysN |
1mM IPTG,4oC,o/n |
loose | Protein QC 4 23 18 |
4/23 | yijia | |||
| 989 | Nico21(DE3)plysN |
1mMIPTG,4oC,o/n |
loose | Protein QC 4 23 18 |
4/23 | yijia | |||
| 947 | Nico21(DE3)plysN |
1mM IPTG,4oC,o/n | loose | Protein QC 4 23 18 |
4/23 | yijia | |||
| 986 | Nico21(DE3)plysN |
1mM IPTG,4oC,o/n |
loose | Protein QC 4 23 18 |
4/23 | yijia | |||
| 945 | Nico21(DE3)plysN |
1mM IPTG,4oC,o/n |
loose | Protein QC 4 23 18 |
4/23 | yijia | |||
| 942 | Nico21(DE3)plysN |
1mM IPTG,4oC,o/n | loose | Protein QC 5 3 2018 |
5/3 | yijia | |||
| 944 | Nico21(DE3)plysN |
1mM IPTG,4oC,o/n | loose | Protein QC 5 3 2018 |
5/3 | yijia | |||
| 3122 | CRM197 |
Nico21(DE3)plysN |
0.25mM IPTG,RT,o/n |
loose | Protein Expression 8 17 2018 |
8/17 | yijia | ||
| 3127 | qBeta-qBeta |
Nico21(DE3)plysN |
0.25mM IPTG,RT,o/n |
loose | Protein Expression 8 17 2018 |
8/17 | yijia | ||
| 984 | SBP-SUMO-3C |
Nico21(DE3)plysN |
0.25mM IPTG,RT,o/n |
loose | Protein Expression 8 17 2018 |
8/17 | yijia | ||
| 3248 | T7-RNAP-P266L |
Nico21(DE3)plysN |
0.25mM IPTG,RT,o/n |
loose | Protein Expression 8 17 2018 |
8/17 | yijia | ||
| 129 | Nico21(DE3)plysN |
0.25mM IPTG,RT,o/n |
loose | Protein Expression 8 17 2018 |
8/17 | yijia |