134 pL42-TthMuts

From ELN
Revision as of 13:16, 14 October 2018 by Crm (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Subcloning:

For 134: vector 212 and  insert 1707: Nco1 + Xho1, wrong, should use Nde1 + Not1

For 136: Vector 2038 and insert  129, Nco1 - Xho1 


RTENOTITLE

Miniprep digestion

RTENOTITLE

Repeat

10/7/2018

Lane 1: 129: Nde`+ Not1

Lane 2: 2038: Nde`-Not1

RTENOTITLE

http://www.excellgen.net/eln/images/3/37/1708_212_3257.JPG

Lane ID Name Enzyme
1 129
Nde1-Not1
2 2038
Nde1-Not1
3 1708
Nco1-Xho1
4 212
Nco1-Xho1
5 3257
EcoR1-Nhe1
6 3260
EcoR1-Nhe1
7 3261
EcoR1-Nhe1
8 3259
EcoR1-Nhe1
9 3258
EcoR1-Nhe1
10 3262
EcoR1-Nhe1

10/9/2018

RTENOTITLE

10/10/2018RTENOTITLE


RTENOTITLE

Retrieved from "https://eln.excellgen.com/index.php?title=134_pL42-TthMuts&oldid=19437"